来源网址：http://www.rsoau.com 2019-10-21 10:59:55
The degradation performance of biodegradable materials and the degradation performance test of biodegradable materials have not been standardized. They can be used as methods, including those adopted or prepared by biochemistry and the American Standard for testing materials (ASTM).
评价微生物实验方法的主要方法如下。 土包埋法有两种：室外土包埋法和室内土包埋法。 微生物来源主要是土壤中的微生物群。 一定时间后，取出样品以测量重量损失，机械性能变化或电子测量。显微镜确定土壤中微生物入侵样品的条件。 优点是可以反映自然环境条件下的生物降解性。 缺点是试验周期长，土壤质量不同，试验结果也不同，重复性差。
The main methods to evaluate microbial experimental methods are as follows. There are two methods of soil embedding: outdoor method and indoor method. The main source of microorganism is the microbial community in the soil. After a certain time, take out the sample to measure the weight loss, mechanical property change or electronic measurement. Microscopes determine the conditions under which microorganisms in the soil invade the sample. The advantage is that it can reflect the biodegradability of natural environment. The disadvantages are long test period, different soil quality, different test results and poor repeatability.
使用陪替氏装置将测试样品和营养琼脂添加到容器中，接种微生物进行培养，并在一定时间后分析样品的重量损失以及一些物理或化学变化。 优点是可以快速降解，短时间内即可得到测试结果，重复性好，定量性能好； 缺点是它不能反映自然界的实际情况。
The test sample and nutrient agar were added to the container with Pettigrew device, inoculated with microorganism for cultivation, and the weight loss of the sample and some physical or chemical changes were analyzed after a certain period of time. It has the advantages of rapid degradation, short time to get the test results, good repeatability, good quantitative performance; the disadvantage is that it can not reflect the actual situation of nature.
酶分析方法是将缓冲液和测试样品添加到容器中，让酶在一定时间内起作用，分析样品的重量损失，目测测量霉菌的生长，并分析其物理性质或化学性质。 通过显微镜检查样品。 优点是测试周期短，重复性好，定量性好。 缺点是它不能反映自然界的实际情况。
Enzyme analysis method is to add buffer solution and test sample to the container, let the enzyme play a role in a certain period of time, analyze the weight loss of the sample, visually measure the growth of mold, and analyze its physical or chemical properties. Examine the sample through a microscope. The advantages are short test cycle, good repeatability and good quantification. The disadvantage is that it can not reflect the actual situation of nature.
放射性C14示踪法使用C14标记聚合物产物，在微生物的作用下生成CO2，在碱性溶液中吸收，通过滴定法测量CO2的总量，然后通过放射性衰变速率法确定C14的CO2量， 使用C14 CO2产生的CO2的百分比表示微生物侵袭的程度。 优点是实验结果可信且清晰。 生物降解性测试可以测试样品生物降解性的优缺点。
The radioactive C14 tracer method uses C14 to label the polymer product, which generates CO2 under the action of microorganism and absorbs it in the alkaline solution. The total amount of CO2 is measured by titration, and then the amount of CO2 in C14 is determined by the radioactive decay rate method. The percentage of CO2 produced by C14 CO2 is used to indicate the degree of microbial invasion. The advantage is that the experimental results are credible and clear. Biodegradability test can test the advantages and disadvantages of sample biodegradability.
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